Fig. 6. Expression of genes involved in renal fibrosis in kidneys of wild-type (WT) and Trpc6^-/- mice. Renal mRNA levels of (A) collagen type 1, alpha1 (Col1α1), (B) collagen type 3, alpha1 (Col3α1), (C) collagen type 4, alpha1 (Col4α1), (D) transforming growth factor beta 1 (TGFβ1) and (E) alpha smooth muscle actin (αSMA) in sham-operated groups and in UUO-operated groups. Renal mRNA expression data were determined in n=5 each for sham-operated WT and Trpc6^-/- mice, n=9 for UUO-operated WT mice and n=8 for UUO-operated Trpc6^-/- mice. The relative standard curve method was used for relative quantification. All data are means ± SD., ns p>0.05, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. ns, not significant.